Journal: Nature Biomedical Engineering

Article Title: A lab-on-a-chip for the concurrent electrochemical detection of SARS-CoV-2 RNA and anti-SARS-CoV-2 antibodies in saliva and plasma

doi: 10.1038/s41551-022-00919-w

Figure Lengend Snippet: ROC curve analysis for electrochemical assays

Article Snippet: A volume of 100 μl of 1 μg ml −1 antigens S1 (SinoBiological, 40591-V08H), N (RayBiotech, 130−10760) and S1-RBD (The Native Antigen Company, REC31849) were prepared in a 10 mM PBS buffer at pH 7.4, added to Nunc MaxiSorp ELISA plates (BioLegend, 423501) and immobilized on the plates by overnight incubation at 4 °C.

Techniques:

Journal: Nature Biomedical Engineering

Article Title: A lab-on-a-chip for the concurrent electrochemical detection of SARS-CoV-2 RNA and anti-SARS-CoV-2 antibodies in saliva and plasma

doi: 10.1038/s41551-022-00919-w

Figure Lengend Snippet: a , Schematic illustrating the multiplexed EC serological assay to assess host antibody responses on electrodes functionalized with SARS-CoV-2 antigens. Host antibodies bind to the SARS-CoV-2 antigens immobilized on the chips. Subsequently, biotinylated anti-human IgG secondary antibodies bind, followed by polystreptavidin-HRP binding and TMB precipitation on the chips. b – e , Typical cyclic voltammograms for the four different electrodes that target host antibodies against S1 subunit ( b ), S1-RBD ( c ), N ( d ), and BSA negative control ( e ). f , ROC curves generated from the patient sample data obtained for the IgG EC serology assay.

Article Snippet: A volume of 100 μl of 1 μg ml −1 antigens S1 (SinoBiological, 40591-V08H), N (RayBiotech, 130−10760) and S1-RBD (The Native Antigen Company, REC31849) were prepared in a 10 mM PBS buffer at pH 7.4, added to Nunc MaxiSorp ELISA plates (BioLegend, 423501) and immobilized on the plates by overnight incubation at 4 °C.

Techniques: Serologic Assay, Binding Assay, Negative Control, Generated

Journal: Nature Biomedical Engineering

Article Title: A lab-on-a-chip for the concurrent electrochemical detection of SARS-CoV-2 RNA and anti-SARS-CoV-2 antibodies in saliva and plasma

doi: 10.1038/s41551-022-00919-w

Figure Lengend Snippet: a , Schematic of the multiplexed chip surface conjugated with SARS-CoV-2 antigens: S1, S1-RBD and N, as well as PNA for the detection of SARS-CoV-2 viral RNA on the LOC microfluidic system. b – e , Current (A) EC readout from the LOC microfluidic chip for a triplicate of clinical samples containing different host antibody and viral RNA combinations: b , Clinical samples negative for both serology and viral RNA. c , Clinical samples with negative host antibody levels but are positive for viral RNA. d , Clinical samples that contain host antibodies against SARS-CoV-2 but are negative for viral RNA. e , Clinical samples positive for both host antibodies and viral RNA. Clinical RNA samples showed clear signal difference between positive and negative clinical samples (Student’s t -test, P = 0.0002) and the clinical IgG showed clear signal difference for all three antigen tests (Student’s t -test for S1 and S1-RBD, P < 0.0001 and for N, P = 0.0004, datapoints represent independent replicates, black lines represent the median value).

Article Snippet: A volume of 100 μl of 1 μg ml −1 antigens S1 (SinoBiological, 40591-V08H), N (RayBiotech, 130−10760) and S1-RBD (The Native Antigen Company, REC31849) were prepared in a 10 mM PBS buffer at pH 7.4, added to Nunc MaxiSorp ELISA plates (BioLegend, 423501) and immobilized on the plates by overnight incubation at 4 °C.

Techniques: